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Laser surgery Radially emitting laser fibre treatment involves using a small laser beam to seal the fistula. SIS promotes tissue remodelling while being slowly incorporated into the body during a 3 to 6-month period. All patients provided written informed consent before participating in the study. Comparison of results fibrin glue anal fistula treatment of fistula-in-ano. Follow-up clinic visits occurred 1 week, 1 month, 3 months, and 1 year after the operation.